Name : Human GGT1 Protein
Product Source :
Recombinant Human GGT1 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Pro27-Tyr569.[Accession | P19440-1]
Molecular Weight :
The protein has a predicted MW of 59.69 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 55-65 kDa (heavy chain) and 25-30 kDa (light chain) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GGT1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GGT1 is greater than 95% as determined by SEC-HPLC.
Background :
Gamma-glutamyl transferase (GGT; EC 2.3.2.2) is the only enzyme capable of degrading glutathione (GSH) in extra-cytosolic spaces. Overexpression of γ-glutamyl transpeptidase (GGT1) has been implicated in an array of human diseases including asthma, reperfusion injury, and cancer.
Synonyms :
Gamma-glutamyltransferase 1; GGT 1; CD224; GGT; GGT-1; GGTD; D22S672; D22S73; D22S732
References & Citations :
(1) Giaretta S, Prasad D, Forieri I, Vamerali T, Trentin AR, Wirtz M, Hell R, Masi A. Apoplastic gamma-glutamyl transferase activity encoded by GGT1 and GGT2 is important for vegetative and generative development. Plant Physiol Biochem. 2017 Jun;115:44-56. doi: 10.1016/j.plaphy.2017.03.007. Epub 2017 Mar 10. PMID: 28319794.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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