Name : Human GIP Protein
Product Source :
Recombinant Human GIP Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu22-Gln93.[Accession | P09681]
Molecular Weight :
The protein has a predicted MW of 9.2 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GIP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GIP is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human GIP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA.
Background :
The potential application of glucose-dependent insulinotropic polypeptide (gastric inhibitory polypeptide, GIP) in the management of obesity and type 2 diabetes has been controversial. Initial interest in the therapeutic use of GIP was dampened by evidence that its insulinotropic activity was reduced in type 2 diabetes and by reports that it increased glucagon secretion and adipose deposition in non-diabetic individuals.
Synonyms :
GIP; Incretin
References & Citations :
(1) Bailey CJ. GIP analogues and the treatment of obesity-diabetes. Peptides. 2020 Mar;125:170202. doi: 10.1016/j.peptides.2019.170202. Epub 2019 Nov 19. PMID: 31756366.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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