Name : Human GHR/Growth Hormone R Protein
Product Source :
Recombinant Human GHR/Growth Hormone R Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala27-Tyr264.[Accession | P10912-1]
Molecular Weight :
The protein has a predicted MW of 28.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human GHR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human GHR is greater than 95% as determined by SEC-HPLC.
Background :
Pegvisomant, a growth hormone receptor (GHR) antagonist, is a well-known drug that was designed to treat acromegaly. However, recent studies have indicated that the GHR is a “moonlighting” protein that may exhibit dual functions based on its localization in the plasma membrane and nucleus.
Synonyms :
GH receptor; GHBP; GHR; GHR/BP
References & Citations :
(1) Lan H, Li W, Li R, Zheng X, Luo G. Endocytosis and Degradation of Pegvisomant and a Potential New Mechanism That Inhibits the Nuclear Translocation of GHR. J Clin Endocrinol Metab. 2019 Jun 1;104(6):1887-1899. doi: 10.1210/jc.2018-02063. PMID: 30602026.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
FGFR-1 alpha (IIIb) Protein
TIE-2 Protein
Popular categories:
Frizzled
P-Selectin/CD62P