Name : Human APOE2/Apolipoprotein E Protein
Product Source :
Recombinant Human APOE2/Apolipoprotein E Protein is expressed from HEK293 with His tag at the C-terminus. It contains Lys19-His317(R176C).[Accession | P02649-1]
Molecular Weight :
The protein has a predicted MW of 35.28 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human APOE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human APOE2 is greater than 95% as determined by SEC-HPLC. SPR Data Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE2, His Tag with an affinity constant of 27.11 nM as determined in SPR assay (Biacore T200).
Background :
Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer’s disease (AD).
Synonyms :
APOE2; APOE; Apo-E
References & Citations :
(1) Zhao N, Liu CC, Qiao W, Bu G. Apolipoprotein E, Receptors, and Modulation of Alzheimer’s Disease. Biol Psychiatry. 2018 Feb 15;83(4):347-357. doi: 10.1016/j.biopsych.2017.03.003. Epub 2017 Mar 14. PMID: 28434655; PMCID: PMC5599322.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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