Name : Human IL-23 alpha&IL-12 beta Protein
Product Source :
Recombinant Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the C-terminus. It contains Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta).[Accession | Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)]
Molecular Weight :
The protein has a predicted MW of 19.77 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 23 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-23 alpha&IL-12 beta, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human IL-23R, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
Background :
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn’s disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.
Synonyms :
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β
References & Citations :
(1)Ma C, Panaccione R, Khanna R, Feagan BG, Jairath V. IL12/23 or selective IL23 inhibition for the management of moderate-to-severe Crohn’s disease? Best Pract Res Clin Gastroenterol. 2019 Feb-Apr;38-39:101604. doi: 10.1016/j.bpg.2019.02.006. Epub 2019 Feb 19. PMID: 31327402.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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