Name : Human Thyroid Peroxidase Protein
Product Source :
Recombinant Human Thyroid Peroxidase Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe19-Arg846.[Accession | P07202-1]
Molecular Weight :
The protein has a predicted MW of 90.6 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Thyroid Peroxidase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Thyroid Peroxidase is greater than 95% as determined by SEC-HPLC.
Background :
Thyroid peroxidase, also called thyroperoxidase (TPO) or iodide peroxidase that encodes a 933 amino-acid residue (aa) molecule with a single membrane-spanning region. Thyroid peroxidase plays a key role in thyroid hormone synthesis by catalyzing both the iodination of tyrosine residues to form monoiodotyrosine (MIT) and diiodotyrosine (DIT) residues and the coupling of iodotyrosine residues in Tg, resulting in the formation of T3 and T4. It is a frequent epitope of autoantibodies in autoimmune thyroid disease, for example, the expression of thyroid peroxidase is lost in papillary thyroid carcinoma.
Synonyms :
TPO; THPO; MGC163194; MGDF; MKCSF; ML; MPLLG; THCYT1; MGDFC-mpl ligand; MK-CSF; MPL ligand; Thyroperoxidase; TPOMKCSF
References & Citations :
(1) Ruf J, Carayon P. Structural and functional aspects of thyroid peroxidase. Arch Biochem Biophys. 2006 Jan 15;445(2):269-77. doi: 10.1016/j.abb.2005.06.023. Epub 2005 Jul 27. PMID: 16098474.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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