Name : Human TPM1 Protein
Product Source :
Recombinant Human TPM1 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Met1-Met284.[Accession | NP_000357.3]
Molecular Weight :
The protein has a predicted MW of 34.26 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TPM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TPM1 is greater than 90% as determined by SEC-HPLC.
Background :
Tropomyosin-1 (TPM1), a widely expressed actin-binding protein, is downregulated in many tumors and associated with cancer progression. TPM1 overexpression in RCC cell lines can induce tumor cell apoptosis via the p53-mediated mitochondrial pathway. Further studies are needed to fully elucidate the potential of TPM1 as a candidate for RCC targeted therapy in the future.
Synonyms :
C15orf13; TMSA; CMD1Y; CMH3; HTM-alpha; LVNC9
References & Citations :
Tang C, Wang J, Wei Q, Du YP, Qiu HP, Yang C, Hou YC. Tropomyosin-1 promotes cancer cell apoptosis via the p53-mediated mitochondrial pathway in renal cell carcinoma. Oncol Lett. 2018 May;15(5):7060-7068. doi: 10.3892/ol.2018.8204. Epub 2018 Mar 7. PMID: 29731872; PMCID: PMC5921034.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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