Name : Mouse Thyroid Peroxidase Protein
Product Source :
Recombinant Mouse Thyroid Peroxidase Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly32-Arg834.[Accession | P35419]
Molecular Weight :
The protein has a predicted MW of 90.6 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse Thyroid Peroxidase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse Thyroid Peroxidase is greater than 95% as determined by SEC-HPLC.
Background :
Thyroid peroxidase, also called thyroperoxidase (TPO) or iodide peroxidase that encodes a 933 amino-acid residue (aa) molecule with a single membrane-spanning region. Thyroid peroxidase plays a key role in thyroid hormone synthesis by catalyzing both the iodination of tyrosine residues to form monoiodotyrosine (MIT) and diiodotyrosine (DIT) residues and the coupling of iodotyrosine residues in Tg, resulting in the formation of T3 and T4. It is a frequent epitope of autoantibodies in autoimmune thyroid disease, for example, the expression of thyroid peroxidase is lost in papillary thyroid carcinoma.
Synonyms :
TPO; THPO; MGC163194; MGDF; MKCSF; ML; MPLLG; THCYT1; MGDFC-mpl ligand; MK-CSF; MPL ligand; Thrombopoietin; TPOMKCSF
References & Citations :
(1) Ruf J, Carayon P. Structural and functional aspects of thyroid peroxidase. Arch Biochem Biophys. 2006 Jan 15;445(2):269-77. doi: 10.1016/j.abb.2005.06.023. Epub 2005 Jul 27. PMID: 16098474.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
PADI2 ProteinAccession
AIF1 ProteinBiological Activity
Popular categories:
PAC1-R
Cadherin-20