Name : Biotinylated Human CD98 Protein
Product Source :
Recombinant Biotinylated Human CD98 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus. It contains Arg206-Ala630.[Accession | AAH01061]
Molecular Weight :
The protein has a predicted MW of 49.75 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human CD98 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Biotinylated Human CD98, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for HAnti-CD98 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
Background :
The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in their affinity for ligand. In this study we explored the role of the transmembrane and cytoplasmic domains of CD98 on integrin-dependent cell adhesion and migration in polarized renal epithelial cells.
Synonyms :
4F2hc; CD98; SLC3A2; 4F2; CD98HC; MDU1; NACAE; 4T2HC
References & Citations :
(1) Cai S, Bulus N, Fonseca-Siesser PM, Chen D, Hanks SK, Pozzi A, Zent R. CD98 modulates integrin beta1 function in polarized epithelial cells. J Cell Sci. 2005 Mar 1;118(Pt 5):889-99. doi: 10.1242/jcs.01674. Epub 2005 Feb 15. PMID: 15713750.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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